HPLC测定藏药风毛菊中茵芋苷、东莨菪苷和伞形花内酯的含量(3)
2.Tibet Autonomous Region Institute for Food and Drug Control， Lasa 850000， China)
[Abstract] This study is aimed to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of skimmin， scopolin and umbelliferone in Saussurea hieracioides. Samples were analyzed on a Wondasil C18-WR column (4.6 mm×250 mm， 5 μm) with methanol (A) and water containing 0.1% phosphate (B) as mobile phases for gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength and column temperature were set at 325 nm and 35 ℃， respectively， and the sample size was 10 μL. The results showed that skimmin， scopolin and umbelliferone were simultaneously achieved within 40 min under the above conditions. A good linearity was observed in the range of 0.18-5.6 μg (r=1.000 0)， 0.060-1.8 μg (r=0.999 9)， 0.032-0.97 μg (r=0.999 8) for skimmin， scopolin and umbelliferone， respectively， with the average recoveries of 99.16% (RSD=0.41% )， 100.3% (RSD=0.79%)， 102.2% (RSD=0.87%). The method is simple， accurate and reproducible and can be used for the quality control of S. hieracioides.
[Key words] Tibetan medicine； Saussurea hieracioides； HPLC； content determination； quality control
doi：10.4268/cjcmm20140620, 百拇医药(谭荣 王毓杰 张雨欣 周礼仕 谭尔 阿萍 张艺)
[Abstract] This study is aimed to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of skimmin， scopolin and umbelliferone in Saussurea hieracioides. Samples were analyzed on a Wondasil C18-WR column (4.6 mm×250 mm， 5 μm) with methanol (A) and water containing 0.1% phosphate (B) as mobile phases for gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength and column temperature were set at 325 nm and 35 ℃， respectively， and the sample size was 10 μL. The results showed that skimmin， scopolin and umbelliferone were simultaneously achieved within 40 min under the above conditions. A good linearity was observed in the range of 0.18-5.6 μg (r=1.000 0)， 0.060-1.8 μg (r=0.999 9)， 0.032-0.97 μg (r=0.999 8) for skimmin， scopolin and umbelliferone， respectively， with the average recoveries of 99.16% (RSD=0.41% )， 100.3% (RSD=0.79%)， 102.2% (RSD=0.87%). The method is simple， accurate and reproducible and can be used for the quality control of S. hieracioides.
[Key words] Tibetan medicine； Saussurea hieracioides； HPLC； content determination； quality control
doi：10.4268/cjcmm20140620, 百拇医药(谭荣 王毓杰 张雨欣 周礼仕 谭尔 阿萍 张艺)